Journal: eLife

Article Title: Registered report: IDH mutation impairs histone demethylation and results in a block to cell differentiation

doi: 10.7554/eLife.10860

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Article Snippet: IDH2 ORF clone , Nucleic acid , OriGene , RC201152 , Replaces Invitrogen plasmid in pOTB7.

Techniques: Bradford Assay, Reporter Assay, Cell Culture, Plasmid Preparation, Generated, Western Blot, Staining, Protease Inhibitor, Protein Concentration

Journal: eLife

Article Title: Registered report: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate

doi: 10.7554/eLife.12626

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Article Snippet: IDH2 R172K ORF in pCMV6 , Plasmid , Origene , RC400103 , .

Techniques: Modification, Plasmid Preparation, Protein Extraction, Protease Inhibitor, Spectrophotometry, DC Protein Assay, Staining

Journal: The Journal of Biological Chemistry

Article Title: Impact of structural biologists and the Protein Data Bank on small-molecule drug discovery and development

doi: 10.1016/j.jbc.2021.100559

Figure Lengend Snippet: A , allosteric inhibitor enasidenib ( red ) targeting homodimeric IDH2 ( yellow and blue monomers ; PDB ID: 5i96 ). B , superposition of apo ( green PDB ID: 5i95 ) and inhibited ( yellow and blue monomers ; PDB ID: 5i96 ) forms of IDH1 computed by overlaying the central domains (boxed in red ). Arrows indicate the locations of the two active sites. The view in ( B ) is rotated ∼45° about the vertical relative to ( A ).

Article Snippet: In 2018, the PDB archive housed six MX structures of human IDH2, all of which were contributed by biopharmaceutical companies ( i.e. , Novartis or Agios).

Techniques: